• 12Feb

    Kat Medical have been the sole distributors for Nidacon for over 10 years now. Nidacon are a Swedish company manufacturing media primarily for purification and storage of sperm for the ART ( assisted reproductive technology ) market and invited distributors and end-users for training on a new product they have developed for use in vitrification.

    On the 19th January 2010 ( after many dramas with the visa application, but that’s subject for another story), I travelled from a hot mine dump strewn Westrand to a very different environment in Gothenburg, on the west Coast of Sweden. Unfortunately there are no direct flights from SA to Sweden so with Turkish Airlines I flew via Istanbuls  Ataturk airport, sounds simple!

    Initially all went according to plan, no delays, minimum stop-over times and to be frank, partly as I was born in Scotland, the weather was to my liking i.e. in the minuses! Snow really does make a city look pretty, a clean ( albeit deep) covering of snow over every surface which appears to brighten up what in a city usually appears dull, roofs and roads. This doesn’t last long in Sweden especially in winter , as the sun rises after 9am and fades from 3pm onwards creating a night time appearance very early in a South Africans bio-rhythm. None the less after 26 hrs of flying, catching trains ( 160km/h plus!) and buses I needed to make good friends with a bed and pillow. I awoke with the sun ( what would we do without that big star) and travelled via taxi to Nidacon facilities for the much anticipated vitrification training. I was truly blown away by the friendly, hospitable and professional environment the staff at Nidacon portray and what a facility they have. The building stands 4 storeys high and the production labs are really a sigh to behold- absolutely immaculate!

    We started training with lunch and from here on I knew this was going to be enjoyable. A small yet cozy group of distributors and embryologists started arriving from a variety of destinations around the globe including Turkey, Norway, Egypt and China.

    The program was eventful and timeous ( in true Swedish fashion i.e. almost to the designated minute ) with a relaxed yet informative variety of speakers sharing their knowledge.

    A splendid supper ended the day after a practical demonstration of the vitrification process by Thorir Hardarson from the fertility centre at Gothenburg.

    Day 2 began with practical training on the density gradient  staining  and cryopreservation techniques using Nidacons  Pure sperm 40/80, and cryprotect reagents. This was very useful and informative and I would like to thank Paul Holmes , Director of Nidacon and all his wonderful staff for a truly  fantastic and educational trip, one day I will return.

    Alas my journey home was not so simple. The snow which was falling from the day I arrived was not abating therefore  take off at the designated time did not occur resulting in the connecting flight from Istanbul to JHB leaving without me. Ah well, I could think of worse places in the world to be stranded than Turkey so I yet again dressed up to resemble a snowman and explored the wonderful city of Istanbul and its many markets.

    Eventually ( 2 days later ) I was happily sitting in my seat bound for JHB reflecting on the vast differences and experiences that had occurred in the past 6 days and I have never before been so keen to see a mine dump as I was landing back on SA soil!

    1. Please feel free to drop me a mail here should any of the above mentioned products be of interest to yourselves or your colleagues and I will respond promptly

    Pictures to follow soon.

    Regards

    Robin McCulloch

    Posted via email from katmedical’s posterous

  • 10Feb

    Vitrification by definition, according to Wikipedia is the process of converting a substance into a glass, however this does not properly explain what the process of vitrification helps with in assisting in fertility methods be it IVF, ICSI or IUI.

    Why Vitrification?

    The main problem when freezing cells is the formation of intracellular ice crystals during both cooling and warming, since these ice crystals have detrimental effect on cell survival. Vitrification, the extremely rapid freezing of cellular materials, makes it possible to freeze cells without forming ice crystals within the cells. The result of vitrification is a very homogenous, amorphous crystalline structure.

    Why Nidacon Vitrification?

    Vitriblast and Thermoblast are based on clinically, well tested standard formulations (lane et al). Numerous publications demonstrate their effectiveness regarding both survival of blastocysts and pregnancy rates.

    Follow-up studies have been done on live-births, where a comparison was made with babies from fresh blastocysts, slow-frozen early cleavage stage embryos and vitrified blastocysts.

    Since the formulisations have been used considerably, Nidacon can provide you with a very detailed protocol for vitrifying blastocysts, just for your security.

    KAT Laboratory is a distributor of the Nidacon Products in South Africa

    For more information on Nidacon products please

    Contact us on 0027 11 472 7300 / sales@katmedical.com

    www.katmedical.com

    Posted via email from katmedical’s posterous

  • 09Feb

    Why is Blood grouping important?

    A person’s blood type becomes a crucial matter when a blood transfusion is necessary. If an individual is transfused with the wrong blood type, a reaction that severely compromises the immune system can result. There are different levels at which a mismatched transfusion can occur, from minor to major incompatibility. Minor factors may lead to very minor problems, but a major incompatibility of blood type during a transfusion can be serious.

    Blood type is so important during a transfusion that donors giving blood to a blood bank must meet certain requirements. A blood transfusion is closely monitored, often by a haematologist.

    In pregnancy…

    Blood tests will check your blood type, your Rh status, and an antibody screen. Your blood type needs to be checked so that a potential mix-up of bloods can be averted if there is a need for a transfusion. Rh negative women need to be identified in advance so that they can be considered as candidates for RhoGAM, an immunoglobulin that is given at 28 weeks of pregnancy and within 72 hours of delivery in order for the mother to keep from becoming Rh sensitized. Occasionally, RhoGAM is also given when there is a potential risk for fetal blood to mix with the mother’s blood, such as in a car accident or in amniocentesis.Why it’s important (RhoGAM): Giving this medication prevents the mother’s body from mounting an antibody response against an Rh positive baby in future pregnancies. An antibody screen detects antibodies, both Rh and less common types, that may occur in the fetus or newborn.

    Why it’s important (antibody screen): Antibodies have the potential for causing blood disease in the fetus and newborn.

    In the case of organ transplants, the tissue of the donor organ must match the recipient’s blood type. A mismatch can cause rejection, and therefore, without a positive match, the transplant will not be conducted. Individuals placed on a transplant waiting list have their blood type thoroughly tested before being placed on the list.

    What are the different blood groups?

    In 1901, scientist Karl Landsteiner reported that blood could be classified into blood ‘types’.

    There are 4 main blood groups A, B, AB and O, of which group O is the most common (47% of population). The blood type is determined by proteins called antigens found on the surface of red blood cells. If you have the antigen A on the red blood cells then you have got type A blood. When B antigen is present, you have type B blood, when both A and B are present, you have type AB blood. When neither are present you have type O blood.

    Another blood group system involves Rhesus factors. The name Rhesus comes from the Rhesus monkeys in which the protein was first discovered. Rhesus factor D, the most important, is found in the blood of 85% of people, they are known as Rhesus positive. The remaining 15% are Rhesus negative. So people can be classified according to both systems, for example AB positive or O negative.

    The Rhesus factor is important during pregnancy, a baby’s life can be endangered if it inherits a Rhesus positive blood type from its father while the mother is Rhesus negative. This is because the mother can form antibodies against the baby’s blood.

    Here is a list of the blood types and their frequency in the general population :

    O Rh (D) Positive 40%
    O Rh (D) Negative 7%
    A Rh (D) Positive 36%
    A Rh (D) Negative 6%
    B Rh (D) Positive 7%
    B Rh (D) Negative 1%
    AB Rh (D) Positive 2.5%
    AB Rh (D) Negative 0.5%

    Eldon card Blood grouping test procedure…

    Step 1

    Place one drop of water in each field with a pipette. DO NOT LET THE WATER DRY.

    Step 2

    Disinfect a finger. Let the finger dry. With a lancet make a small puncture.

    Step 3

    Collect one drop of blood for each field on an ELDONstick®. Use a new ELDONstick® for each field.

    Step 4

    Blood drops should fit the base of the ELDONstick®.

    Step 5

    Place the blood drops into the fields of your ELDONCARD®. Keep the stick in contact with the card.

    Step 6

    Mix blood and reagent with a rotating movement for approx. 15 seconds. Spread mixture to the perimeter of the circles.

    Step 7

    Tilt the ELDONCARD® to a vertical position. After 10 seconds tilt the ELDONCARD® 180° to allow blood to travel across the reaction fields. Wait 10 seconds. Tilt the ELDONCARD® 90° to the left – wait 10 seconds. Tilt the ELDONCARD® 180° to the right – wait 10 seconds. Read and record the result. Let the ELDONCARD® dry (approx. 2 minutes if you use a hairdryer).

    Step 8

    Cover the agglutinations with adhesive foil to prevent deterioration of reactions and to seal possible infectious material.

    KAT Laboratory is a distributor of the eldon card system in Southern Africa

    For more information on the Eldon Card Blood grouping please

    Contact us on 0027 11 472 7300 or sales@katmedical.com

    www.katmedical.com

    Posted via email from katmedical’s posterous

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